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  1. Products
  2. qPCR & PCR
  3. Custom qPCR probes
21_NG_ProductOverview_HeaderIcon_qPCR

Custom qPCR probes

Use custom probes to leverage the broad range of sequence design, dye, and purification options available from IDT.

PrimeTime™ qPCR Probes

PrimeTime qPCR probes are customized to your specifications and include several probe types, including PrimeTime double-quenched probes, Affinity Plus LNA Probes, and molecular beacon probes. Additionally, MGB Eclipse® Probes (EliTech Group) and other probe types are available as GMP-compliant reagents.

*MGB Eclipse is a registered trademark of ELITech Group
** GMP refers to product manufactured in accordance with IDT’s ISO 13485: 2016 QMS

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Affinity Plus™ qPCR Probes

Use Affinity Plus qPCR Probes for enhanced discrimination of thermodynamically similar samples such as single nucleotide polymorphisms and transcript variants. The Affinity Plus bases used in these qPCR probes include up to 6 locked nucleic acid monomers. When incorporated into a probe, locked nucleic acids impart heightened structural stability, leading to increased hybridization melt temperature (Tm).

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PrimeTime™ LNA® qPCR Probes

PrimeTime LNA qPCR probe sequence (10–25 standard bases with up to 6 locked nucleic acid nucleotides), reporter, and quencher. Choose from a wide variety of reporter and quencher combinations.

LNA is a registered trademark of Qiagen.

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Molecular beacon probes

Molecular beacons are dual-labeled probes with self-complementary ends that form a quenched, hairpin structure in the absence of the target nucleic acid sequence. Signal is generated by linearization of the probe upon binding to the target sequence during PCR cycling. Use molecular beacon probes to identify single-base mismatches and for high-precision applications.

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MGB Eclipse® Probes

MGB Eclipse probes and companion primers are manufactured under ISO 13485 conditions, which can help you transition from research to clinical diagnostics.

MGB Eclipse is a registered trademark of ELITech Group.

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GMP Primers and Probes

DNA oligos manufactured under GMP. Fast, easy, and clear process to determine the best products to meet your specific needs for more rigorous quality requirements.

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Overview

qPCR probe design and production quality are paramount in creating specific qPCR assays with robust signal-to-noise performance. IDT qPCR probes are HPLC purified to remove free residual dye and truncated synthesis products that can contribute to unwanted background signal.

Good probe design must balance the need for high affinity with considerations for best signal generation and quenching. We have a variety of options to address even complex design requirements. Generate powerful data with custom probes from IDT by leveraging our state-of-the-art oligonucleotide manufacturing, proprietary quenchers, and choice of license-free dyes.

GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing.

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