Antibodies are a key element of any immune system, but they also have a lot of uses for research applications. The following article describes the structure and function of antibodies, which is essential knowledge for researching biologics, antigen tests, and using them as a tool in Western blots, chromatin immunoprecipitation, and immunofluorescence.
Antibodies are a specific type of protein. These specialized Y-shaped proteins bind to antigens, which can stimulate an immune response (Figure 1).
Today, antibodies are utilized in antigen testing kits and biologics. Antibodies are also an integral tool used in several research methods:
Antibodies are secreted into the blood and mucosa, which facilitates three biological functions:
Antibodies are made up of two identical light chains (yellow) and two identical heavy chains (blue) (Figure 2).
Light chains: Have one variable domain (VL) and one constant domain (CL).
Heavy chains: Have one variable domain (VH) and three or four constant (CH) domains.
These variable domains are encoded by DNA. There are a countless number of combinations of these regions to create a diverse pool of antibodies with highly specific antigen-binding sites. Finding the most effective antigen-binding sequences can be used to research potential therapies and/or new diagnostic tests.
The difference between light and heavy chain is that the light chain is the small polypeptide subunit of an antibody while the heavy chain is the large polypeptide unit of an antibody.
There are five types of heavy chain constant regions found in antibodies. Each antibody type is classified as IgG, IgM, IgA, IgD, or IgE. A summarized list of each antibody type and known function is described in Table 1.
Table 1. The five types of antibodies
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