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Nuclease detection and control

Reagents for rapid detection and elimination of RNases and DNases. The detection assays, which are effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified using fluorometry. Two oligonucleotide substrates are offered for nuclease detection, RNaseAlert® and DNaseAlert™ substrates. Both are available in a variety of configurations:

  • Complete kit—includes single use tubes, buffer, water, enzyme, and Nuclease Decontamination Solution
  • Single-use tubes—25 single-reaction tubes ideal for rapid, hand-held, visual assays at the bench
  • Bulk tubes—for use in visual assays or quantitative measurement in a fluorometer

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Nucleases are widely present in research labs and can interfere with experiments that use nucleic acids. Single-stranded RNases are particularly ubiquitous, hard to eliminate, and can rapidly degrade important samples or reagents used in genomics research.

Nuclease detection

RNaseAlert and DNaseAlert Substrates allow for rapid, sensitive detection of RNases and DNases. These in-house formulated reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescent signal only after nuclease degradation. The assays can be read visually for qualitative assessment of contamination or by fluorometry for a quantitative measurement. Qualitative measurements are useful for testing lab reagents, equipment, and supplies for nuclease contamination. The assays can be used quantitatively to study enzyme kinetics. The RNaseAlert Substrate employs a FAM™ reporter (Em 520 nm), while the DNaseAlert Substrate employs a HEX™ reporter (Em 555 nm).

Figure 1. Examination of RNase-free sample versus RNase-positive control.

RNaseAlert Kit components

  • 25 x 50 pmol Fluorescent Substrate tubes
  • 250 µL RNaseAlert Buffer
  • 2 mL Nuclease-Free water
  • 50 µL RNase A
  • 50 mL Nuclease Decontamination Solution

DNaseAlert Kit components

  • 25 x 50 pmol Fluorescent Substrate tubes
  • 250 µL DNaseAlert Buffer
  • 2 mL Nuclease-Free water
  • 25 µL 2U/uL DNase I Enzyme
  • 50 mL Nuclease Decontamination Solution

Nuclease control

Use Nuclease Decontamination Solution on and around your workspace to irreversibly inactivate nucleases and reduce contamination risk. It eliminates the need to bake glassware, and can be applied to plastic surfaces which are often difficult to sterilize. Just spray, rinse and let dry, then confidently carry out your experiments.

User guides and protocols

Technical reports