- Tried and true products—curated for targeted methylation sequencing
- Library preparation from low input—input as low as 5 ng
- Powered by xGen Adaptase™ technology—template independent adapter attachment reduces workflow time while generating a less biased library
- Cost effective and reliable—key targets are enriched and sequenced deeply
- Comprehensive sample representation—uniform methylome genome coverage by targeted bisulfite sequencing with low bias
- Flexible and customizable—allows for custom design of oligos
- Workflow design for easy automation
What is targeted methyl-seq?
Methylation sequencing (methyl-seq), also referred to as bisulfite sequencing is a next generation sequencing (NGS) approach that researchers use to study methylation patterns of DNA. These patterns can be used to identify different cell types in epigenetics
and oncology research [1,2]. Researchers can study the methylation status of the entire genome using whole genome bisulfite sequencing (WGBS) or target specific regions of the genome using hybridization panels to enrich genomic regions of interest.
More specifically, a targeted methyl-seq approach (also called targeted bisulfite sequencing) uses hybridization probes to capture and enrich biologically relevant regions of interest but with deeper sequencing depth and higher coverage than WGBS.
Targeted methyl-seq workflow
A targeted methyl-seq workflow provides researchers with a streamlined procedure for targeted methylation sequencing (also called targeted bisulfite sequencing). The xGen Methyl-Seq DNA Library Prep Kit has a post-bisulfite library preparation workflow
to maximize library complexity by converting bisulfite-induced single-stranded fragments directly into library molecules. This kit is compatible with a range of input quantities and library molecules can be generated in as little as 2 hours with bisulfited-converted
The addition of an xGen Custom Hyb Panel to this workflow enables researchers to capture biologically relevant regions of the genome for targeted methyl-seq. When used for targeted methyl-seq, the xGen Custom Hyb Panel results in sequences with a high on-target
mapping percentage and efficiency (Figure 1), as well as methylation profiles that are highly correlated to those obtained from WGBS (Figure 2). Further details on the xGen Custom Hyb Panel for targeted methyl-seq can be found in the AppNote, Cost-effective targeted methyl-seq using an xGen Custom Hyb Capture Panel and the xGen Methyl-Seq DNA Library Prep Kit identifies DNA methylation in low input samples consistent with WGBS.
The recommended group of xGen methyl-seq product offerings also include the compatible core reagents the xGen Hybridization and Wash Kit, xGen Universal Blockers TS, and xGen Library Amplification Primer Mix to accomplish methyl-seq with an easy and flexible workflow.
Together, these xGen NGS products offer you a comprehensive workflow for accelerating your epigenetic research using a targeted methylation sequencing approach.