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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Why is my methylation sequencing run exhibiting low Q-scores?
Bisulfite conversion results in low complexity libraries since they are C-depleted, which may require a high complexity spike-in such as PhiX library to avoid low Q-scores.
Contact Illumina if you need specific recommendations on sequencing bisulfite converted samples for your sequencing instrument.