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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Where on a nucleotide are Cy® dyes attached?
5′ Cy dyes are attached to the 5' hydroxyl group of the sugar via a phosphodiester bond.
3' Cy dyes are attached to the 3' hydroxyl group of the sugar via a phosphodiester bond.
Internal Cy dyes are attached to the backbone via the phosphodiester bonds of the bases between which the dyes occur.