What reverse transcription (RT) module is recommended?
The xGen™ SARS-CoV-2 Amplicon Panel used with the xGen Amplicon Core Kit and indexing primers supports first- or second-strand cDNA, generated from the SARS-CoV-2 RNA genome, as input.
Select a cDNA synthesis module that supports two-step RT-PCR, includes random primers, and has a processivity of >1 kb.
The panel has been tested with Superscript® IV First-Strand Synthesis System (Thermo Fisher Cat. No. 18091050). The manufacturer’s protocol was followed as written using random primers and associated specifications. Steps involving the optional host gDNA/RNA removal and RNAse H were not performed.