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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What is the optimal amount of Alt-R HDR Donor Blocks to use with my cell type and what can I do to prevent toxicity and/or cell death after delivering Alt-R HDR Donor Blocks?
Due to recognition of cytosolic dsDNA by innate immune response pathways, careful consideration of the final Alt-R™ HDR Donor Block concentration is necessary to avoid cytotoxicity. We recommend testing for optimal concentrations for each cell line you use, as sensitivity to dsDNA may vary. To learn more, go to the Alt-R Donor Blocks protocol.