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What are the N and K mixed bases that can be incorporated into the variable regions of gBlocks Gene Fragments?
Amino acids are represented by 1–4 codons that are defined by the genetic code. NNK codons are commonly used in screens for codon substitutions to reduce the presence of some codon-rich amino acids, thereby reducing their overrepresentation in a particular library.
Additionally, using NNK codons removes 2 out of 3 possible stop codons, which also limits the number of sequences in a library that produce unwanted truncated gene products.