Should I amplify my gBlocks™ HiFi Gene Fragments when I receive them?
gBlocks HiFi Gene Fragments are clonal products and can be amplified if necessary. However, most applications such as restriction-digest, blunt-end, and Gibson cloning can be performed with the material provided by IDT, and PCR amplification should not be necessary.
If the purpose of the amplification is to add flanking bases to complete a cloning reaction, we strongly recommend ordering the gBlocks HiFi Gene Fragment (IDT) with the required bases rather than adding them afterwards using PCR.
If you choose to amplify your gBlocks HiFi Gene Fragment, use the minimum amount of starting template required by your PCR kit protocol, limit the cycle number to 10–12, and use a high-fidelity polymerase.