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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is the PrimeTime Gene Expression Master Mix appropriate for qPCR assays that use intercalating dyes (e.g., SYBR® Green and EvaGreen® dyes)?

No. PrimeTime™ Gene Expression Master Mix is compatible with probe-based assays (such as all PrimeTime qPCR Assays from IDT and TaqMan® Assays from Thermo Fisher Scientific). 

SYBR is a registered trademark of Life Technologies, Inc.
EvaGreen dye is a registered trademark of Biotium.