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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How many freeze-thaw events can PrimeTime™ One-Step RT-qPCR Master Mix withstand?
We tested up to 15 freeze-thaw cycles, which is more extreme than typical laboratory usage. No detrimental effects or loss in Cq were observed. However, we still recommend following good laboratory practice by minimizing freeze-thaw cycles.