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We do not recommend using PCR to add the overhangs, because PCR can preferentially amplify smaller byproducts in the prep, leading to issues with the cloning reaction and increasing the number of colonies you need to screen to find the correct full-length insert. Instead, build the overhangs into the gBlocks Gene Fragment itself. The amount of material you will receive is enough for a couple of assembly reactions.
The additional PCR step is not required to generate additional material and adds an additional enzymatic step to the protocol that can introduce errors.
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