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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Do I resuspend fluorescently labeled oligos the same as I would standard, unlabeled oligos?
We strongly recommend resuspending and storing fluorescently labeled oligos in a buffered solution such as TE (10 mM Tris pH 8.0, 0.1 mM EDTA) at ‒20°C. This will help increase the shelf life of your dye labeled oligos.
Also, avoid unnecessary freeze-thaw cycles by preparing a freezer stock solution (100 µM) and several working aliquots. Use the IDT Resuspension Calculator under the SciTools™ tab of the IDT website for help determining volumes to use.