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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Do annealed double-stranded oligos need to be modified prior to ligation into the appropriate restriction site of my cloning vector?
Our chemical synthesis results in a hydroxyl group on both the 3' and 5' terminus of the oligo. DNA ligase requires a 5' phosphate and a 3' hydroxyl group for activity, so a 5' phosphate will need to be added if the intent is to ligate the oligo.
This can be requested upon order using the modification code /5Phos/ in the sequence of your oligo. Alternatively, this modification can be added enzymatically through a Polynucleotide Kinase (PNK) reaction.