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Yes. This technology can convert the short, single-stranded fragments, common in ancient DNA, into NGS library molecules.
However, if the ancient DNA sample is known to have uracil-containing DNA, the xGen Methyl-Seq DNA Library Prep Kit should be used because it can handle uracil-containing DNA while the xGen ssDNA & Low-Input DNA Library Prep Kit is not able to handle uracil-containing DNA.
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