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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Why is purification not recommended for randomized oligos?
Additional purification for oligonucleotides incorporating randomized bases can result in a bias in the degeneracy of the randomized bases.
Oligos with degenerate/randomized bases do not migrate in a tight distribution. Purification by either method (PAGE or HPLC) may purify out some of the randomized sequences. This results in the reduction of sequence diversity.