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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What type of analysis can be performed using the rhAmpSeq™ CRISPR Analysis Tool?
In singleplex analysis, the sample library has only one amplicon that is used to assess the editing of a single site in a sample. You would use singleplex analysis to assess numerous guide RNAs for on-target activity.
In multiplex analysis, the sample library is generated by multiplex PCR and is composed of multiple amplicons that assess the editing on multiple sites in a sample. You would use multiplex analysis to assess the on- and off-target editing of a guide RNA.