Your product is now available from Integrated DNA Technologies.
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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What concentrations are the primers and probe in the Predesigned Primetime™ qPCR Sequences and how are they shipped?
The Mini scale is available at a fixed amount of 1 nmol primers (each) and 0.5 nmol probe. The Standard (500–20 µL reactions) and XL (2500−20 µL reactions) scales have options for primer-to-probe ratio (ranging from 1:1 to 4:1) and a variety of dye/quencher combinations are available.
Standard final concentrations in a qPCR assay are 500 nM for each primer and 250 nM for each probe.