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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What are the advantages of an arrayed CRISPR screen over a pooled screen?
Unlike pooled libraries, knowing exactly which gene was targeted in a population of cells negates the need for a sequencing-based deconvolution step. Additionally, arrayed libraries are amenable to phenotypic-based high-throughput screens because there are no confounding effects from multiple gene knockouts.
Synthetic arrayed gRNA libraries can be delivered directly to cells without viral packaging, avoiding any biosafety issues posed by using lentiviruses.