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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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On your website, I see that there are two different extinction coefficients for various dyes. Which one should I use?
The extinction coefficient "at maximum absorbance" refers to the extinction value that a given molecule has at its unique absorption maximum.
The extinction coefficient "not at absorbance maximum" refers to the extinction coefficient at 260 nm, which is the wavelength used to determine optical density.
See the DECODED article about quantifying your oligo here.