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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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If the target area does not contain a protospacer adjacent motif (PAM) sequence, will the CRISPR-Cas9 complex bind?
No, CRISPR-Cas9 complexes poorly, or not at all, recognize targets lacking PAM sequences .
The PAM sequence recognized by the S. pyogenes CRISPR-Cas9 system is NGG. If this sequence is not present in your target, you may be able to use other CRISPR systems (from other bacterial species) that recognize different PAM sequences. The following table lists examples of PAM sequences: