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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Do the universal terminal sequences or modifications on Alt-R HDR Donor Blocks integrate or affect insertion rates?
When the Alt-R™ HDR Donor Blocks are incorporated via the homology-directed repair (HDR) pathway, we have not observed evidence of integration of the universal terminal sequences, or modifications. The modification reduces unwanted non-homologous insertion (blunt integration) events. However, if undesired blunt integration does occur then the universal terminal sequences may be integrated into the genome along with the donor template, but that would be considered a rare event.