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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Do I need to worry if my shipment of PrimeTime™ Gene Expression Master Mix is delayed?
No. We have tested the PrimeTime Gene Expression Master Mix by heating at 50°C for 1, 3, or 7 days before use in qPCR experiments. In the assays tested (HPRT and GUSB), high PCR efficiency was observed
(range of 95.1–103.6% with R2 >0.991).
When compared to controls (frozen and unheated master mix), ΔCq values were <0.5, with a few exceptions that were primarily seen when using a low amount of starting template. The small ΔCq values emphasize the stability of PrimeTime Master Mix after extreme thermal stress.