Can I use HEX, TET, and 6-FAM labeled primers in my multiplex qPCR?
Although HEX and TET are both chemical derivatives of Fluroescein
, each has its own specific absorbance and emission wavelength characteristics. With properly calibrated equipment, each signal should be easily distinguishable.
If your equipment does not have dye specific filters or cannot be dialed in to a specific wavelength, then you should choose dyes with wavelength spectra that are further apart.