21_NG_ProductOverview_HeaderIcons_SynBio-GeneFragments

Introducing Rapid Genes NEW

Fast genes are in our DNA

Ready-to-use, NGS-verified clonal genes, perfect for quickly moving into your high throughput screening experiments.

Quality you can trust, industry leading speed you’ve come to count on.

Ordering

  • Rapid Genes ship as soon as 5 business days
  • NGS-verified, clonal perfect gene synthesis
  • Available in cloning and expression vectors
  • Plate formats for high throughput workflows

Rapid Genes Gene Synthesis

1 The time required to manufacture a gene is dependent on length, complexity, and vector choice. Sequences that are unstable and/or toxic to E. coli can substantially affect delivery times.

Table 1. Available formats of Rapid Genes.

Plate typeBuffer optionVolumeConcentration
96-well PCR PlateNuclease-free water or IDTE pH 8.050 µL20 ng/µL
Dry (no buffer)N/AN/A
384-well PCR PlateNuclease-free water or IDTE pH 8.050 µL20 ng/µL
384-well Echo™ Plate*Nuclease-free water or IDTE pH 8.050 µL20 ng/µL

To achieve the fastest possible shipping time, items that do not meet IDT's purity and yield specifications after the first assembly attempt will be cancelled at no charge. No formal cancellation notice will be sent for these items. However, cancellations will be noted on the item specification sheet, which can be accessed using the Synthetic Biology Order Status Checker. Plates with less than 24 genes will incur an additional fee applied at checkout.

*Echo is a trademark of Labcyte Inc. a Beckman Coulter company

For Genes longer than 2kb or custom vectors order here.

Product details

IDT Rapid Genes are NGS-verified, circular double-stranded DNA in a plasmid. They range in size from 125bp – 2000bp and ship as soon as 5 business days. They are available in a variety of cloning and expression vectors and ideal for high‑throughput screening workflows.

Table 2. Vectors for Rapid Genes.

Vector nameSelection markerApplicationSequence
pUCIDT (Amp)AmpicillinCloningTXT, GB, PDF
pUCIDT (Amp) Golden GateAmpicillinCloningTXT, GB, PDF
pUCIDT (Kan)KanamycinCloningTXT, GB, PDF
pUCIDT (Kan) Golden GateKanamycinCloningTXT, GB, PDF
pIDTSmart (Amp)AmpicillinCloningTXT, GB, PDF
pIDTSmart (Kan)KanamycinCloningTXT, GB, PDF
pEXP-IDTAmpicillinMammalian ExpressionTXT, GB, PDF
pET-IDTKanamycinBacterial ExpressionTXT, GB, PDF
pET-IDT C HisKanamycinBacterial ExpressionTXT, GB, PDF
pET-IDT Dual His + ThrombinKanamycinBacterial ExpressionTXT, GB, PDF
pET-IDT N His + ThrombinKanamycinBacterial ExpressionTXT, GB, PDF

They are available to ship dry or in resuspension buffer in 96-well or 384-well plates.

Table 3. Rapid Genes characteristics.

SpecificationInformation
Length 125bp-2000bp
QC 100% NGS verification
Shipping Time* 5-8 business days
Yield 1µg
Format Dry or resuspended in buffer at 20 ng/µl

* Shipping time refers to the number of business days after order confirmation (excluding Fridays for wet plates). Shipping time is dependent on length and complexity of the Rapid Genes ordered. In a few cases, shipping time may exceed the estimated time.

Icons_Ocean_85x85_Support

Request a consultation

Have questions for our experts? Want to onboard your own vector? Your time is valuable and we’ll prioritize your inquiry. Click on “Request a consultation” to provide brief information about your project, and we’ll be in touch to discuss it ASAP.

Request a consultation

Product data

Sequence verification

All inserts for Rapid Genes are sequence-verified by NGS. Upon delivery of the plasmids, an NGS-sequencing report, plasmid map, and FASTA file are all available to you in your online IDT account.

NGS QC example, depth of coverage-percent wild type

Sample NGS report

Frequently asked questions

Biosecurity

Sequence Information is secure and confidential at IDT. Please see our Confidentiality Statement for more information. All online ordering steps, including sequence entry and your choice of parameters, are also secure and protected.

We screen the sequence of every gene, gene fragment, and Megamer™ ssDNA fragment order we receive to (1) identify any regulated and other potentially dangerous pathogen sequences, and (2) verify that IDT’s gene customers are legitimate scientists engaged in beneficial research.

IDT is among the five founding members of the International Gene Synthesis Consortium (IGSC) and helped to create the IGSC’s Harmonized Screening Protocol. The Harmonized Screening Protocol describes the gene sequence and customer screening practices that IGSC member companies employ to prevent the misuse of synthetic genes. IDT takes the steps set out in the Harmonized Screening Protocol to screen the sequences of ordered genes and the prospective customers who submit those orders.

For more information about the IGSC and the Harmonized Screening Protocol, please visit the website at www.genesynthesisconsortium.org.

logo_igsc

In October 2010, the United States government issued final Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA, describing how commercial providers of synthetic genes should perform gene sequence and customer screening. IDT and the other IGSC member companies supported the adoption of the Screening Framework Guidance, and IDT follows that Guidance in its application of the Harmonized Screening Protocol. For more information, please see 75 FR 62820 (Oct. 13, 2010), or https://federalregister.gov/a/2010-25728.

RUO24-2871_001